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codelink activated microarray slide  (Danaher Inc)


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    Danaher Inc codelink activated microarray slide
    Codelink Activated Microarray Slide, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codelink+activated+microarray+slide/pm25414728-227-12-16
    Average 86 stars, based on 1 article reviews
    codelink activated microarray slide - by Bioz Stars, 2026-09
    86/100 stars

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    Article Title: A DNA-based pattern classifier with in vitro learning and associative recall for genomic characterization and biosensing without explicit sequence knowledge.
    Article Snippet: Each sample in the well plate was printed with 10 replications onto CodeLinkTM activated microarray slide (Amersham Biosciences) using MicroGrid II microarray printing system with BioRobotics MicroSpot 2500 pins (Genomic Solutions, MI) at 40% relative humidity according to the previously established protocol [17].



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    Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by <t>DNA</t> <t>microarrays</t> competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.
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    Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by <t>DNA</t> <t>microarrays</t> competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.
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    Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by DNA microarrays competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.

    Journal: PLoS Pathogens

    Article Title: Genome-Wide Fitness Test and Mechanism-of-Action Studies of Inhibitory Compounds in Candida albicans

    doi: 10.1371/journal.ppat.0030092

    Figure Lengend Snippet: Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by DNA microarrays competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.

    Article Snippet: We custom-built a set of two DNA microarrays using Amersham CodeLink Activated Slides ( http://www.gelifesciences.com/ ).

    Techniques: Amplification, Labeling